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Georgia's Metropolitan Atlanta snappily Transit Authority uses IBM Watson IoT apparatus to champion predict, identify and restore abilities issues with faultfinding device and machinery
ARMONK, N.Y., Feb. 28, 2019 /PRNewswire/ -- IBM (NYSE: IBM) these days announced a brand original portfolio of web of issues (IoT) solutions that team synthetic intelligence (AI) and superior analytics to champion asset intensive corporations, such because the Metropolitan Atlanta quick Transit Authority (MARTA), to expand renovation concepts. The solution is designed to champion companies to lessen expenses and in the reduction of the possibility of failure from actual property equivalent to motors, manufacturing robots, turbines, mining machine, elevators, and electrical transformers. IBM Maximo Asset efficiency management (APM) options assemble statistics from physical belongings in near precise-time and supply insights on existing operating conditions, prognosticate talents concerns, determine complications and offer restoration recommendations.
IBM)" alt="IBM these days introduced a original portfolio of web of things options, IBM Maximo Asset performance administration, bringing together synthetic intelligence and advanced analytics to assist asset intensive organizations, such as the Metropolitan Atlanta rapid Transit Authority (MARTA) in Georgia, to enhance preservation innovations. The solution collects records in immediate real-time from any physical asset, reminiscent of vehicles, manufacturing robots, mills, mining machine, elevators, electrical transformers and extra, an d provide insights on present working situations, predicts competencies considerations, identifies complications and presents restore techniques. (IBM)" />
businesses in asset-intensive industries like energy and utilities, chemical compounds, oil and gas, manufacturing, and transportation, can tolerate thousands of belongings that are Important to operations. These assets are more and more producing great quantities of information on their working circumstances. holding these assets up and running is essential to operations and monitoring and optimizing property on-going maintenance, fix, and substitute selections requires regular evaluation. in response to analyst arduous Aberdeen research, unplanned downtime can can pervade a corporation as much as $260,000 an hour.
company leaders can now recognize if a laptop is probably going to fail, the exhaust of advanced analytics and AI to retract into account timing, prognosticate consequences and establish issues. IBM's APM solutions champion companies shift asset renovation thoughts from preventative to predictive and prescriptive with the aid of integrating disparate information sources to discover property in requisite of attention and recommending actions.
This acknowledge enhances a corporation's current trade asset management (EAM) capabilities, reminiscent of IBM's market leading Maximo EAM solution, and integrates seamlessly with other EAM suppliers. It contains:
Asset fitness Insights: provides asset fitness assessments in near real-time the usage of asset information, sensor facts, and other external information to notify upkeep and replacement choices.
Predictive renovation Insights: Predicts asset health using statistical fashions and computing device gaining knowledge of. comprises failure date/likelihood, key drivers, degradation curves, and anomaly detection.
gadget administration Assistant: makes it possible for technicians to restoration machine with an AI-powered helper presenting faster access to documentation, diagnostics and suggestions for restoration
IBM will additionally present the APM suite customized for specific industries, beginning with APM for power and Utilities (E&U). This provides business-selected capabilities to research and act on insights from utility belongings and contains risk/criticality scoring, health and degradation fashions, common trade information mannequin, and weather facts integration.
"it's essential for businesses to believe about how helpful their renovation practices are. With the launch of its original Asset efficiency management solutions, IBM is assisting companies contour perception-pushed selections with an integrated portfolio of superior analytics and AI capabilities to assist them enrich operational effectiveness and efficiency," pointed out Kareem Yusuf, Ph.D., universal manager, IBM Watson IoT. "Maximo APM is designed to enhance a firm's existing enterprise Asset administration gadget by artery of addressing complete points of asset performance together with fitness, predictive maintenance and restore optimization."
MARTA keeps Atlanta, Georgia running
The Metropolitan Atlanta speedy Transit Authority (MARTA), the foremost public transit agency in the Atlanta metropolitan enviornment, is working with IBM to reclaim in accommodate a predictive preservation acknowledge to enhance reliability of property, minimize expenses and create a Transit Asset management (TAM) device that offers asset inventory, situation assessment, efficiency measures and choice support. through data mining, computer researching and AI, MARTA can entry and resolve statistics to enhanced bethink the circumstance of machine classified in the classes of life defense, operation faultfinding and operation champion to determine potential issues of a "gadget" with assorted stakeholders. subsequently, the acknowledge will allow MARTA to seamlessly circulation from monitoring asset efficiency KPIs to predicting and fighting asset failures.
"MARTA is on course to revolve into the primary North American public transit company to obtain ISO 55000 certification. taking section with IBM gives MARTA with the innovation from a expertise icon, which fortifies us as an industry chief in Transit Asset management," talked about Remy Saintil, director of facilities at MARTA.
About IBMIBM options are designed to allow customers to expand the operational efficiency of their physical belongings and reduce fees during the power of IoT information and synthetic intelligence. by using amassing and curating statistics from billions of related contraptions, sensors, and methods international, they empower companies to derive original insights from their equipment, automobiles and amenities. IBM's trade knowledge, leading protection, and options constructed for a hybrid cloud atmosphere firmly establish us as a leader in IoT. For extra assistance on IBM Watson IoT, gladden talk over with www.ibm.com/iot.
IBM corporation emblem. (PRNewsfoto/IBM)
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No outcomes discovered, are attempting original key phrase!2018 IBM dividend hike: 4.7% 2017 dividend hike: 7.1% 2016 dividend hike: 7.7% That quickly payout boom from MSFT is courtesy of a vastly superior trade mannequin and expand trajectory (greater on this ...
well, that took slightly a long time. After what seems like eons of nudging and cajoling and pushing, IBM is making the IBM i operating apparatus and its built-in database administration gadget, as well as the application construction apparatus and other programs application, attainable on its self-branded IBM Cloud public cloud.
massive Blue previewed its plans to bring each IBM i and AIX to the IBM Cloud at its annual suppose conference in Las Vegas, on scale out machines aimed toward miniature and medium corporations as well as to clients who want to exhort clusters of machines, and on scale up programs that tolerate NUMA electronics that more tightly cluster them into shared memory techniques.
There are a lot of questions about how this might be complete be packaged up and bought under the unwieldy cognomen of the IBM vigour methods digital Server on IBM Cloud. however they are able to let you know complete that they understand and fill you in as they gain knowledge of greater.
The verve programs slices served up on the IBM Cloud operating IBM i and AIX tolerate been first rolled out for early entry to chosen valued clientele to check the APIs for the handle plane, which they presume is in keeping with the PowerVC implementation of OpenStack. The preliminary customers who're kicking the tires had been putative to simplest installation evolution and test environments or protype code, not at complete the repose working in production. This was absolutely putative to be a proof of concept setup at this point, as you could notice from the rollout roadmap:
The alpha and beta trying out for the energy programs slices on the IBM Cloud is on a trustworthy looking quickly music, operating from February into March, as which you could see, and commonplace availability is slated for a while in March. doubtless somewhere towards the end, they would wager. IBM is at the beginning making the power puffs – what else are you able to cognomen them as shorthand? – obtainable in the early entry exams in its US East datacenter in the Washington DC metro area; it isn't lucid what iron turned into used. The alpha and beta testing is being completed on a group of vigour S922 scale out machines, which can be in response to a pair of Power9 processors and which frankly is a great box for many IBM i stores complete with the aid of itself, as well as on energy E880 scale up methods, that are being scaled as much as sixteen sockets and which exhaust the older Power8 processors. complete of the machines exhaust ten-core processors, which are the sweet spot between efficiency, chip yield, and price within the energy techniques line. The Power9 chips within the energy S922 servers exhort at 2.8 GHz and might turbo as much as three.9 GHz if there is satisfactory thermal headroom; these chips issue to be they may tolerate four threads per core (often known as SMT4), which isn't as a gross lot threading as the ordinary Power8 chip offers in scale out machines with SMT8 threading (eight threads per core) and twice as many cores per die (24 cores versus 12 cores). Anyway, it seems like the power S922 is being equipped with the massive cores, now not the mild ones. The Power8 chips within the power E880 are operating at 4.19 GHz and additionally sheperd SMT8 threading (there was only a massive core right here); complete sixteen cores are populated with ten-core Power8 chips, with a maximum scalability of 160 cores in a single analytic partition on the IBM Cloud.
each of the machines are being configured with 32 GB memory sticks, which can be once again at the sweet spot between means, bandwidth, and price per bit, and IBM is allowing valued clientele to configure the machines with anywhere from 8 GB to 64 GB of leading reminiscence per core. if you requisite denser memory to bolster the skill, that you would be able to double it up the exhaust of sixty four GB memory sticks, and then double it up again to 128 GB sticks, however this may be a custom order and it will can pervade significantly extra, they consider. IBM is providing disk and glimmer storage as much as 2 TB for aboriginal storage on the situations and growing to be at 10 GB increments. external storage for applications and databases is on Storwize V7000 disk arrays in the racks immediate this vigour iron, and are linked to the servers via Fibre Channel switches. valued clientele can share machines or employ dedicated iron, reckoning on their proclivities for absolute security and coping with uproarious neighbors on the digital infrastructure.
Steve Sibley vp and offering supervisor for the Cognitive programs division at IBM, gave me a sneak peek at the future energy cloud slices on a talk over with to the Austin, Texas labs final week, forward of the believe convention. here is how the facets and functions of the analytic partitions on the IBM Cloud are shaping up:
Sibley told us that the pricing changed into now not yet set in stone for the digital energy capacity on the IBM Cloud, but when pressed about evaluating the cost between buying apparatus and renting it on the cloud, Sibley estimated that for a given IBM i or AIX workload running on in a similar artery configured hardware and analytic partitions, running the cloud instance would pervade about 30 p.c to 40 p.c extra on the illustration working full out over three years compared to buying the iron and the exhaust of it for three years. The difference, of direction, is that IBM is managing the verve systems and purchasing the datacenter, the electricity, the cooling, the security, patch updates on firmware, the installing of the PowerVM hypervisor and its replace, and the setting up of the IBM i or AIX working device. When organizations purchase their own energy systems machines, until they accept augmented features from IBM or a enterprise companion, it’s complete on them. Pricing for the digital machines, the reminiscence, the storage, and the working apparatus and databases are complete set per hour.
incidentally, it isn't lucid what IBM i and AIX releases are supported on the machines, but most likely the existing IBM i 7.2 and IBM i 7.three releases are supported on the IBM Cloud as well as AIX 7.1 and AIX 7.2, that are furthermore present. Linux isn't mentioned as a supported platform, but SAP HANA workloads are and that means that there is a Linux – and above complete red Hat enterprise Linux – beneath it considering that HANA in-reminiscence databases are not supported on any Unix variant, or IBM i for that remember.
by means of March, IBM plans to tolerate the IBM i and AIX instances purchasable in creation in its US East (Washington DC) and US South (Dallas) regions, and in the 2d quarter IBM plans to roll it in its European datacenter in its eu imperative (Frankfurt) area, with different datacenters coming out as exact pulls and IBM pushes.
we are able to retract a glance on the pricing as soon because it turns into obtainable and spot how it complete stacks up, and accept the suggestions of alternative IBM i cloud avid gamers about what huge Blue is doing.
Public Cloud Dreaming For IBM i
Skytap Says It’s edifice a ‘authentic Cloud’ offering for IBM i
a far better approach to skin The IBM i Cloud Cat
What Cloud providers should upshot To entice IBM i
Google near Launching IBM i Cloud service, Will Says
Is The ‘Golden Age’ of Computing Leaving IBM i at the back of?\
Ensono Emerges With IBM i Cloud providing
Clouds grow, however Can IBM i observe?
IBM ultimately receives Power8 Machines On SoftLayer Cloud
All signals point To The Cloud, however Will IBM i throng comply with?
mobile Apps And The IBM i concern manufacturing unit
Infor Readies IBM i Cloud
IBM i Finds a situation in the Cloud
vigour programs Coming To The SoftLayer Cloud
IBM Brags About Its Cloud Prowess due to SoftLayer
IBM Buys SoftLayer To build Out hosting, Cloud agencies
Two quiz TPM Questions: precise IBM i Clouds And Apple buying IBM i
IBM i 7.1 Tech Refresh activities live Partition Mobility
AWS/four hundred: Amazon Builds An AS/four hundred-oid Cloud
wanted: Cloud-i i-nfrastructure
The X factor: Head within the Clouds
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Feb 28, 2019 (Heraldkeeper via COMTEX) -- Global Airport Digitization Industry
New Study On "2018-2025 Airport Digitization Market Global Key Player, Demand, Growth, Opportunities and Analysis Forecast" Added to sapient Guy Reports Database
This report focuses on the global Airport Digitization status, future forecast, growth opportunity, key market and key players. The study objectives are to present the Airport Digitization evolution in United States, Europe and China.
In 2017, the global Airport Digitization market size was million US$ and it is expected to compass million US$ by the conclude of 2025, with a CAGR of during 2018-2025.
The key players covered in this studyCisco SystemsIBMMicrosoftSiemensSITAAppleScarabeeWind RiverDaifukuLiving PlanIT
Market segment by Type, the product can be split intoHardwareSoftwareService
Market segment by Application, split intoPassenger Screening and SecurityBaggage ServicesPassenger AssistanceOther
Market segment by Regions/Countries, this report coversUnited StatesEuropeChinaJapanSoutheast AsiaIndiaCentral & South America
The study objectives of this report are:To resolve global Airport Digitization status, future forecast, growth opportunity, key market and key players.To present the Airport Digitization evolution in United States, Europe and China.To strategically profile the key players and comprehensively resolve their evolution blueprint and strategies.To define, record and forecast the market by product type, market and key regions.
For minute Reading gladden visit WiseGuy Reports @ https://www.wiseguyreports.com/reports/3552470-global-airport-digitization-market-size-status-and-forecast-2018-2025
Some Major Points from Table of content:
1 Report Overview1.1 Study Scope1.2 Key Market Segments1.3 Players Covered1.4 Market Analysis by Type1.4.1 Global Airport Digitization Market Size Growth Rate by ilk (2013-2025)1.4.2 Hardware1.4.3 Software1.4.4 Service1.5 Market by Application1.5.1 Global Airport Digitization Market share by Application (2013-2025)1.5.2 Passenger Screening and Security1.5.3 Baggage Services1.5.4 Passenger Assistance1.5.5 Other1.6 Study Objectives1.7 Years Considered
2 Global Growth Trends2.1 Airport Digitization Market Size2.2 Airport Digitization Growth Trends by Regions2.2.1 Airport Digitization Market Size by Regions (2013-2025)2.2.2 Airport Digitization Market share by Regions (2013-2018)2.3 Industry Trends2.3.1 Market Top Trends2.3.2 Market Drivers2.3.3 Market Opportunities
3 Market share by Key Players3.1 Airport Digitization Market Size by Manufacturers3.1.1 Global Airport Digitization Revenue by Manufacturers (2013-2018)3.1.2 Global Airport Digitization Revenue Market share by Manufacturers (2013-2018)3.1.3 Global Airport Digitization Market Concentration Ratio (CR5 and HHI)3.2 Airport Digitization Key Players Head office and locality Served3.3 Key Players Airport Digitization Product/Solution/Service3.4 Date of Enter into Airport Digitization Market3.5 Mergers & Acquisitions, Expansion Plans
4 Breakdown Data by ilk and Application4.1 Global Airport Digitization Market Size by ilk (2013-2018)4.2 Global Airport Digitization Market Size by Application (2013-2018)
5 United States5.1 United States Airport Digitization Market Size (2013-2018)5.2 Airport Digitization Key Players in United States5.3 United States Airport Digitization Market Size by Type5.4 United States Airport Digitization Market Size by Application
6 Europe6.1 Europe Airport Digitization Market Size (2013-2018)6.2 Airport Digitization Key Players in Europe6.3 Europe Airport Digitization Market Size by Type6.4 Europe Airport Digitization Market Size by Application
7 China7.1 China Airport Digitization Market Size (2013-2018)7.2 Airport Digitization Key Players in China7.3 China Airport Digitization Market Size by Type7.4 China Airport Digitization Market Size by Application
8 Japan8.1 Japan Airport Digitization Market Size (2013-2018)8.2 Airport Digitization Key Players in Japan8.3 Japan Airport Digitization Market Size by Type8.4 Japan Airport Digitization Market Size by Application
9 Southeast Asia9.1 Southeast Asia Airport Digitization Market Size (2013-2018)9.2 Airport Digitization Key Players in Southeast Asia9.3 Southeast Asia Airport Digitization Market Size by Type9.4 Southeast Asia Airport Digitization Market Size by Application
10 India10.1 India Airport Digitization Market Size (2013-2018)10.2 Airport Digitization Key Players in India10.3 India Airport Digitization Market Size by Type10.4 India Airport Digitization Market Size by Application
11 Central & South America11.1 Central & South America Airport Digitization Market Size (2013-2018)11.2 Airport Digitization Key Players in Central & South America11.3 Central & South America Airport Digitization Market Size by Type11.4 Central & South America Airport Digitization Market Size by Application
12 International Players Profiles12.1 Cisco Systems12.1.1 Cisco Systems Company Details12.1.2 Company Description and trade Overview12.1.3 Airport Digitization Introduction12.1.4 Cisco Systems Revenue in Airport Digitization trade (2013-2018)12.1.5 Cisco Systems Recent Development12.2 IBM12.2.1 IBM Company Details12.2.2 Company Description and trade Overview12.2.3 Airport Digitization Introduction12.2.4 IBM Revenue in Airport Digitization trade (2013-2018)12.2.5 IBM Recent Development12.3 Microsoft12.3.1 Microsoft Company Details12.3.2 Company Description and trade Overview12.3.3 Airport Digitization Introduction12.3.4 Microsoft Revenue in Airport Digitization trade (2013-2018)12.3.5 Microsoft Recent Development12.4 Siemens12.4.1 Siemens Company Details12.4.2 Company Description and trade Overview12.4.3 Airport Digitization Introduction12.4.4 Siemens Revenue in Airport Digitization trade (2013-2018)12.4.5 Siemens Recent Development12.5 SITA12.5.1 SITA Company Details12.5.2 Company Description and trade Overview12.5.3 Airport Digitization Introduction12.5.4 SITA Revenue in Airport Digitization trade (2013-2018)12.5.5 SITA Recent Development12.6 Apple12.6.1 Apple Company Details12.6.2 Company Description and trade Overview12.6.3 Airport Digitization Introduction12.6.4 Apple Revenue in Airport Digitization trade (2013-2018)12.6.5 Apple Recent Development12.7 Scarabee12.7.1 Scarabee Company Details12.7.2 Company Description and trade Overview12.7.3 Airport Digitization Introduction12.7.4 Scarabee Revenue in Airport Digitization trade (2013-2018)12.7.5 Scarabee Recent Development12.8 Wind River12.8.1 Wind River Company Details12.8.2 Company Description and trade Overview12.8.3 Airport Digitization Introduction12.8.4 Wind River Revenue in Airport Digitization trade (2013-2018)12.8.5 Wind River Recent Development12.9 Daifuku12.9.1 Daifuku Company Details12.9.2 Company Description and trade Overview12.9.3 Airport Digitization Introduction12.9.4 Daifuku Revenue in Airport Digitization trade (2013-2018)12.9.5 Daifuku Recent Development12.10 animated PlanIT12.10.1 animated PlanIT Company Details12.10.2 Company Description and trade Overview12.10.3 Airport Digitization Introduction12.10.4 animated PlanIT Revenue in Airport Digitization trade (2013-2018)12.10.5 animated PlanIT Recent Development
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Figure 2 shows a schematic representation of the study design. Sixteen hale manlike BALB/c mice were assigned to one of four radiopharmaceutical groups. Intraperitoneal (i.p.) administration of 15 mg/kg DOX was applied in complete groups every three weeks for one, two, three, or four cycles, respectively. The maximum cumulative dose comprised 60 mg/kg given over 12 weeks, which corresponds to a potentially cardiotoxic dose of 1050 mg per cycle for a 70-kg manlike patient10. At baseline and after completing each cycle of DOX, the cardiac and other organ biodistribution of the four radiopharmaceuticals was investigated approximately one hour after intravenous (i.v.) tail vein administration. After a group of four mice completed the regimen, the left ventricular (LV) role was evaluated by transthoracic echocardiography. Then animals were sacrificed for non-imaging assessment, which included deoxynucleotidyl transferase biotin-dUTP nick-end labeling (TUNEL) staining, cardiac expression of Bcl-2, p53, caspase 3, caspase 8, and HIF-1α and Δψm by Western blot (WB) analysis, immunohistochemistry (IHC), and fluorescence-activated cell sorting (FACS)/flow cytometry. Furthermore, a group of four hale BALB/c mice per radiopharmaceutical served as controls, as they were treated at the same time-points and conditions with saline administration. They were furthermore imaged after every cycle, but were only sacrificed after completing complete four saline cycles. The study was approved by the animal keeping committee of the University of Pisa in accordance with pertinent animal keeping guidelines.
Schematic study design. Non-imaging markers are WB, IHC, and FACS expression markers. † = sacrifice of mice.
Radiopharmaceutical preparation and administration
The lipophilic cation Sestamibi (Cardiolite, Bristol-Myers Squibb) was radiolabeled by adding water to 1–3 ml of sodium pertechnetate (99mTcO4)Na and heated for ten minutes at 100 °C. After cooling, a monovalent lipophilic cationic tangled was retrieved (99mTc-sestamibi-6–2-metossi-isobutyl-isonitril), of which an tolerable of 18.5 MBq in 0.1 mL was injected i.v.
Human recombinant Annexin V (National Institutes of Health, Bethesda, MD, USA) was radiolabeled through combined in vitro incubation at elbowroom temperature for 30 minutes of 60–90 μg di-HYNIC-Annexin V diluted in 0.5 ml of physiological saline solution, 0.74–1.11 MBq pertechnetate anion (99mTcO4−), which was freshly eluted by a 6 GBq generator (Amersham, GE HealthCare), 10–20 μg of reduction agent (stannous chloride dehydrate, SnCl2−2H2O), and 1.5 mg of tricin (N-[tri(hydroxymethyl)methyl]glycine) as co-ligand. Labeling succumb was 85–90%. 99mTc-Annexin V was then injected in a 0.1 mL solution containing on tolerable 18.5 MBq of 99mTc-Annexin V, which corresponds to a total protein concentration of 4.5 ng per animal.
99mTc-glucaric acid (Molecular Targeting Technologies, Inc. West Chester, PA, USA) was prepared from a lyophilized kit containing 12.5 mg of D-glucaric acid and 0.15 mg of stannous chloride11 by adding 1480 MBq (40 mCi) freshly eluted 99mTc-pertechnetate as previously described12. Labeling efficiency (always >98%) was checked by ascending chromatography. The radiopharmaceutical was injected in 0.1 mL solution, containing on tolerable 18.5 MBq 99mTc-glucaric acid.
The glucose analogue [18F]FDG was provided as a bulk vial (Gluscan, GE Healthcare), from which a sample of 18.5 MBq in 0.1 ml was injected. To avoid the intake of carbohydrates, the mice that received [18F]FDG were provided a fat-enriched diet.
LV role was evaluated with transthoracic echocardiography before the mice were euthanized, approximately 10 minutes after initiation of sedation. M-mode images were used for measurements of LV end-diastolic internal diameter (LVEDD) and LV end-systolic internal diameter (LVESD) in two consecutive beats. Fractional shortening (FS) was calculated as FS% = [(LVEDD − LVESD)/LVEDD] × 100.
Biodistribution and histological analysis
After completion of their regimen and one hour after injection of the radiopharmaceuticals, animals were anesthetized and sacrificed. For biodistribution studies, samples of thyroid, kidney, tail, bone, lung, heart, muscle, blood, stomach, skin, liver, spleen, intestine, and urine were removed and weighed immediately. Radioactivity of these samples was registered for 180 seconds by a gamma counter (2470 Automatic Gamma Counter Wizard, PerkinElmer, Waltham, MA, USA), along with an aliquot of the injective and the tail to reform for partial extravasation, expressed as a percentage of the injected dose per gram of tissue (%ID/g).
Before gamma counting, two three-millimeter cardiac tissue slices were obtained for histology: one was frozen at −80 °C in dehydrate ice and the other was placed in 4% formalin. After treatment with a protease inhibitor (1:100 in 10 mM Tris buffer, pH 8), apoptotic cells were assessed with direct fluorescence fragment conclude labeling of DNA breaks (TUNEL kit, Fluorescein FragEL, Calbiochem, San Diego, CA, USA). Caspase 3 and HIF-1α were immunostained using antihuman-mouse lively antibodies (rabbit anti-caspase 3 and rabbit anti-HIF polyclonal antibody, Chemicon International, Temecula, CA, USA) after incubation with a fluorescent-labeled secondary antibody (Alexa Fluor 488 goat anti-rabbit IGg, Thermo Fisher Scientific, Waltham, MA, USA). Cells stained with TUNEL, caspase 3, and HIF-1α were counted in ten randomly selected high-power tumor fields (magnification x200) and expressed as a percentage of total cells. Furthermore, cardiac samples were analyzed by Western blotting to identify the specific amount of Bcl-2, p53 (both Santa Cruz Biotechnology Inc., Dallas, TX, USA), caspase 3, and caspase 8 (both Cell Signaling Technologies Inc., Danvers, MA, USA).
Measurement of Δψm
Inner Δψm was measured by a single fluorescent lipophilic cation 5,5′,6,6′-tetrachloro- 1,1′,3,3′-tetraethylbenzimidazolocarbocyanine iodide (JC-1) that marks the loss of potential. For this purpose, mitochondria were isolated from myocardial tissue using a dedicated kit (Mytocondria Isolation Kit, Sigma-Aldrich, St. Louis, MO, USA) stained with JC-1 and analyzed by FACS (MACSQuant, Miltenyi Biotec, Bergisch Gladbach, Germany). JC-1, concentrated in the mitochondrial matrix of established cells due to the electrochemical potential gradient, forms red fluorescent aggregates. Any event that dissipates the mitochondrial membrane potential prevents the accumulation of the JC-1 dye in the mitochondria. Accordingly, red stained cells depict intact mitochondria, whereas when the dye is dispersed throughout the entire cell, a shift from red (J-aggregates) to green fluorescence (JC-1 monomers) is observed. A red-to-green (i.e., intact to disrupt mitochondrial) ratio was performed and described as upper and lower rim (UR and LR). Consequently, a decreasing ratio was considered a disintegration of membrane potential.
Biodistribution and immunofluorescent data are expressed as weigh in and median %ID/g tissue and standard aberration (SD). Data were analyzed by non-parametrical tests (Kruskal-Wallis and Dunn) to evaluate the differences in groups at different time points and by Mann-Whitney U to test the significance between DOX-treated and DOX-naïve mice. Furthermore, Spearman’s rho was used to assess correlations. Significance was set at p < 0.05. Statistics were performed using IBM SPSS 20 for Windows. The datasets generated and/or analyzed as section of the current study are available from the corresponding author by reasonable request.
The general Services Administration granted IBM a six-month $16 million extension on its contract to operate and modernize the SAM.gov system as the agency considers whether to reclaim the trade up for bidding.
The extension was announced in a contracting document dated Aug. 13 that cited an exigent requisite to preserve IBM on the job while the agency explores the possibility of a competitive offering.
IBM has held the System for Award Management Architecture and Operations contract Services contract for eight years, and it is leading the exertion to modernize the system of systems that government agencies and vendors exhaust to manage hundreds of billions in government contracts annually.
IBM's contract expired Aug. 14. GSA had intended to award IBM another long-term deal, but according to contracting documents, that scoot was overturned because of a ruling that more market research needs to be conducted to champion a determination to warrant a sole source award.
The agency touted IBM's rating in a recent performance assessment.
"IBM's performance is solid and continues to ameliorate over the course of this contract. They tolerate moved from the satisfactory/good even to the very good/excellent even in many areas. Their processes tolerate matured. Their knowledge base continues to grow. IBM continues to confederate with the government to overcome challenges and displays tenacious commitment to program success," the document stated.
GSA is in the midst of a major modernization of the SAM ecosystem, which touches every aspect of civilian and defense contracting, from solicitation to tracking spending. The exertion has been in progress since 2010, and the late pace of the project has generated some ire on Capitol Hill, most notably from Sen. Claire McCaskill (D-Mo.), who threatened to hold up confirmation of Denise Turner Roth to lead GSA in 2015 in order to obtain answers to questions about the cost and timing of the project.
In September 2017, GSA launched a beta site that will eventually encompass nine fully modernized systems, eliminating the requisite for standalone sites such as FedBizOpps, the Federal Procurement Data System and others. So far, just one legacy system -- the Catalog of Federal Domestic Assistance -- has been retired.
Once the modernized system moves from beta to production, GSA anticipates more opportunities, including multiple miniature trade awards to be made for the operation, maintenance and enhancement of the original system.
About the Author
tag Rockwell is a senior staff writer at FCW, whose beat focuses on acquisition, the Department of Homeland Security and the Department of Energy.
Before joining FCW, Rockwell was Washington correspondent for Government Security News, where he covered complete aspects of homeland security from IT to detection dogs and rim security. Over the eventual 25 years in Washington as a reporter, editor and correspondent, he has covered an increasingly wide array of high-tech issues for publications like Communications Week, Internet Week, Fiber Optics News, tele.com magazine and Wireless Week.
Rockwell received a Jesse H. Neal Award for his toil covering telecommunications issues, and is a graduate of James Madison University.
Click here for previous articles by Rockwell. Contact him at email@example.com or succeed him on Twitter at @MRockwell4.
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