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250-510 exam Dumps Source : Administration of SymantecTM Data Loss(R) Prevention 10.5 - BETA

Test Code : 250-510
Test cognomen : Administration of SymantecTM Data Loss(R) Prevention 10.5 - BETA
Vendor cognomen : Symantec
: 196 real Questions

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Symantec Administration of SymantecTM Data

When To cognomen The Feds For trade secret Theft Investigations | killexams.com real Questions and Pass4sure dumps

Law360 (February 5, 2019, 1:forty two PM EST) -- an organization suspects that an worker, a former worker, or a manufacturing or construction companion has stolen its constructive trade secrets. What can and may the industry do?

whereas many organizations...


Symantec Endpoint Encryption: replete disk encryption product overview | killexams.com real Questions and Pass4sure dumps

here's Part of a series on the fine replete disk encryption products and outfit available in the market. For greater, check out their FDE product roundup.

Symantec Endpoint Encryption offers replete disk encryption (FDE) capabilities for the inside tough drives of pcs, laptops and servers. FDE is the means that ensures every bit of difficult drive records is encrypted so elegant records saved thereon cannot exist accessed via an attacker, as long as the computer, computer or server is powered off or (in the case of a desktop) is in a hibernate state. Symantec Endpoint Encryption additionally comprises storage encryption for removable media, comparable to USB scintillate drives and exterior challenging drives.

Product models

The Symantec Endpoint Encryption product became first launched in October 2014, changing the Symantec pressure Encryption product. not relish another companies, Symantec makes a solitary edition of its Endpoint Encryption utility.

it is intended to exist centrally managed by means of the Symantec Endpoint Encryption administration Server product, which should exist hosted in an lively listing area.

Platform support

considering the fact that Symantec Endpoint Encryption is a modern product, it is simply supported on incredibly modern windows working outfit (OS) types, as follows: Microsoft windows eight.1 commercial enterprise and expert, Microsoft home windows eight enterprise and professional, Microsoft windows 7 premiere, industry and expert, Microsoft windows Server 2012 Datacenter and normal, and Microsoft home windows Server 2008 commercial enterprise and standard.

Encryption and authentication support

Symantec Endpoint Encryption helps using the superior Encryption regular (AES) encryption algorithm with either 128-bit keys or 256-bit keys. AES is a robust algorithm and, when used with the 256-bit key option, offers mighty insurance policy in opposition t present and rising threats.

Being a fresh product, Symantec Endpoint Encryption has no longer yet been Federal tips Processing common (FIPS) a hundred and forty-2-certified, but the validation manner is in progress. FIPS a hundred and forty-2 certification shows that a product has been independently tested to determine if it has any giant usual complications in its cryptographic implementation. Certification does not testify that a product is foolproof, but fairly that favorite weaknesses in its cryptography don't exist.

Multifactor authentication is supported by using Symantec Endpoint Encryption, along with integration with lively listing services. The FDE product can employ cryptographic tokens and sage playing cards as sorts of authentication besides passwords.

Symantec Endpoint Encryption presents self-carrier password healing for clients, in addition to advocate desk-provided password recovery. Some companies don't permit self-carrier on account of the relative ease in compromising it via gregarious engineering assaults. The aid desk-supplied alternative gives these corporations one other means of providing a recovery role devoid of the haphazard inherent in self-carrier.

one other beneficial authentication feature that Symantec Endpoint Encryption presents is mitigation of brute-drive authentication assaults, customarily involving passwords. If there are too many failed authentication attempts in a row on a appliance -- with the quantity determined by means of the administrator -- the device can exist configured to instantly add delays between every authentication effort, as a result slowing down an attacker. Or the outfit can exist configured to lock out the user and require the consumer to employ both the self-carrier password healing feature or the advocate desk-provided recuperation choice to regain entry to the system.

administration

As outlined above, Symantec Endpoint Encryption is designed for centralized administration by means of the Symantec Endpoint Encryption administration Server product. This competence it's meant for commercial enterprise employ and not for employ by means of people or by smaller organizations that carry out not result centralized administration.

Licensing

Symantec sells copies of Symantec Endpoint Encryption through its website and through channel partners. As with different industry FDE solutions, Symantec licenses its product via device (e.g., computing device, laptop, server).

right here is the retail pricing as of November 2014.

Symantec presents a free affliction of Symantec Endpoint Encryption.

Conclusion

Symantec Endpoint Encryption offers FDE and detachable media encryption capabilities for more recent home windows programs, pcs, laptops and servers. It isn't a workable commercial enterprise-broad alternative for agencies operating Mac OS X desktops and laptops, neither is it proper for organizations operating older types of windows on computers, laptops and servers.

Symantec Endpoint Encryption’s authentication, encryption and centralized administration capabilities are on par with competing commercial items. since the product has its own committed administration server, it doesn't matter when it comes to integration no matter if the commercial enterprise adopting it already has Symantec items installed. In different words, there is not any potential for existing Symantec purchasers as adversarial to customers of different carriers.


Symantec warns of China-based mostly espionage thrust focused on satellites | killexams.com real Questions and Pass4sure dumps

A China-based mostly cyber neighborhood is accomplishing an extensive hacking crusade by focused on satellite, telecom and defense corporations in the united states and Southeast Asia, a U.S. cybersecurity enterprise warned this week.

The motive of the hacking group, called "Thrip," is likely country wide cyber espionage, safety researchers at Symantec Corp. referred to on Tuesday.

"Thrip’s assault on telecoms and satellite operators exposes the probability that the attackers might intercept or even alter communications traffic from organisations and patrons," Symantec pointed out in a press release, adding that essentially the most worrying discovery is an try to manage satellites by route of infecting linked computers with malware.

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"The assault community gave the print to exist peculiarly interested in the operational side of the company, trying to find and infecting computer systems running software that monitors and controls satellites. this implies to us that Thrip’s motives proceed past spying and can too embrace disruption," Symantec wrote in a separate blog keep up.

Satellites play a primary role in telecommunications, receiving and transmitting telephone and information superhighway alerts as well as mapping and geolocation data.

Symantec pointed out in Tuesday's statement that it has tracked Thrip in view that 2013 and lately observed that the cyber community seems to occupy "found out modern outfit and thoughts used by using the community in this most recent set of attacks."

The danger of the improved hacking toolbox is extra compounded through Thrip's competence to remain well hidden.

"They operate very quietly, mixing in to networks, and are most efficient organize out the employ of ersatz intelligence that can determine and flag their movements," Symantec Chief executive Greg Clark said in a statement.

The enterprise says it organize out the modern assaults via its synthetic intelligence-primarily based targeted assault Analytics, which helped the researchers spot the malicious behavior that become hiding in legit IT administration equipment.


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Administration of SymantecTM Data Loss(R) Prevention 10.5 - BETA

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Dengue virus NS1 triggers endothelial permeability and vascular leak that is prevented by NS1 vaccination | killexams.com real questions and Pass4sure dumps

A leak in the dike

Everyone knows how mosquitos can wreck an end-of-summer picnic. But in some climates, these pesky intruders persist and carry a variety of detrimental diseases—some with no preventative vaccines or targeted therapies. One such passenger is dengue virus (DENV), which infects up to 400 million people each year and comes in several serotypes (1 to 4) and disease presentations—from mild infection to ascetic disease and sometimes death. But to deal or preclude dengue requires that they occupy a more complete picture of the disease pathology. Now, Modhiran et al. and Beatty et al. narrate the results of in vitro and in vivo experiments that point to circulating dengue virus non-structural protein 1 (NS1) and the inborn immune Toll-like receptor 4 (TLR4) as a focus for basic scientists as well as vaccine and drug developers.

DENV infection protects a patient from future reinfection with the identical DENV serotype as well as producing temporary immune protection from ascetic dengue disease caused by a different DENV serotype. But unlike diamonds, this immune protection doesn’t eventual forever, and when the protected age passes, the patient becomes at increased risk of enhanced infection and progression to ascetic disease if he or she is infected with a second DENV serotype. This ascetic figure of dengue infection is believed to result from immunopathogenic processes that induce cytokine storm and occasions vascular leakage that leads to shock. Until now, no dengue viral proteins occupy been linked to vascular endothelium permeability (that is, vascular leakage).

Beatty et al. note that inoculation of mice with DENV NS1 protein lonely induces both vascular leak and secretion of inflammatory cytokines and that administration of NS1 with a sublethal dose of DENV2 leads to lethal vascular leak syndrome. In human endothelial cell monolayers in culture, NS1 from any of the four DENV serotypes triggered endothelial barrier permeability. NS1’s pathogenic effects were blocked by NS1-immune polyclonal mouse serum or monoclonal antibodies to NS1 (in vivo and in vitro), and immunization of mice with NS1 protected against lethal DENV2 challenge.

In an independent study, Mondrian et al. explore the underlying of NS1’s effects. They note that highly purified NS1 acts as a pathogen-associated molecular pattern (PAMP) that activates mouse macrophages and human peripheral blood mononuclear cells (PBMCs) in culture via TLR4, resulting in release of inflammatory cytokines—an outcome that was blocked by either a TLR4 antagonist or an anti-TLR4 antibody. Then, in an in vitro model of vascular leak, the authors organize that NS1 fractured the integrity of endothelial cell monolayers through a TLR4-dependent pathway, a finding that was supported by the observation that a TLR4 antagonist quelled capillary leak in a mouse model of dengue virus infection.

Together, these modern findings highlight NS1 as an instigator of dengue-associated vascular leak and thus pinpoint a potential target for dengue drugs and component for dengue vaccines.

Abstract

The four dengue virus serotypes (DENV1 to DENV4) are mosquito-borne flaviviruses that occasions up to ~100 million cases of dengue annually worldwide. ascetic disease is thought to result from immunopathogenic processes involving serotype cross-reactive antibodies and T cells that together induce vasoactive cytokines, causing vascular leakage that leads to shock. However, no viral proteins occupy been directly implicated in triggering endothelial permeability, which results in vascular leakage. DENV nonstructural protein 1 (NS1) is secreted and circulates in patients’ blood during acute infection; lofty levels of NS1 are associated with ascetic disease. They note that inoculation of mice with DENV NS1 lonely induces both vascular leakage and production of key inflammatory cytokines. Furthermore, simultaneous administration of NS1 with a sublethal dose of DENV2 results in a lethal vascular leak syndrome. They too demonstrate that NS1 from DENV1, DENV2, DENV3, and DENV4 triggers endothelial barrier dysfunction, causing increased permeability of human endothelial cell monolayers in vitro. These pathogenic effects of physiologically germane amounts of NS1 in vivo and in vitro were blocked by NS1-immune polyclonal mouse serum or monoclonal antibodies to NS1, and immunization of mice with NS1 from DENV1 to DENV4 protected against lethal DENV2 challenge. These findings add an notable and previously overlooked component to the causes of dengue vascular leak, identify a modern potential target for dengue therapeutics, and advocate inclusion of NS1 in dengue vaccines.

INTRODUCTION

Dengue virus (DENV) is a mosquito-borne flavivirus that is estimated to occasions up to 390 million infections, 96 million cases of dengue, and ~500,000 hospitalizations annually (1). Infection with any of the four DENV serotypes (DENV1, DENV2, DENV3, and DENV4) results in a ambit of syndromes from inapparent infection to classic dengue fever (DF) to dengue hemorrhagic fever/dengue shock syndrome (DHF/DSS), which is characterized by vascular leakage and shock (2). Most primary DENV infections caused by any of the four serotypes are asymptomatic or lead to the self-limiting but debilitating DF; however, secondary infections with a different (heterologous) DENV serotype can lead to increased risk of ascetic dengue (3). Immune responses after primary DENV infection lead to protective immunity to homologous secondary infection but may either protect against or occasions increased disease severity in a subsequent DENV infection with a different serotype. The latter is thought to exist mediated by serotype cross-reactive T cells or antibody-dependent enhancement (ADE), whereby cross-reactive antibodies that target viral structural proteins facilitate DENV infection of Fcγ receptor–bearing cells, leading to increased viral load (4, 5). ADE and cross-reactive T cells are thought to trigger an exaggerated and skewed immune response to a previously infecting serotype, resulting in a “cytokine storm”—rapid-onset, high-level production of proinflammatory cytokines, including tumor necrosis factor–α (TNF-α) and interleukin-6 (IL-6), in the blood that leads to endothelial permeability and vascular leak (6). However, the potential role of viral proteins in mediating vascular leakage has not been demonstrated.

The DENV 10.7-kb, positive-strand RNA genome encodes a polyprotein that is posttranslationally cleaved by host and viral proteases into three structural proteins [capsid, membrane, and envelope (E)] and seven nonstructural proteins. DENV nonstructural protein 1 (NS1) is a glycosylated 48-kD protein that plays a role in both viral replication and immune evasion (7, 8). NS1 is initially translated as a monomer that is glycosylated in the endoplasmic reticulum (ER) but rapidly forms a dimer that can occupy four fates: association with the viral replication knotty on the surface of the ER membrane, plasma membrane association via a glycophosphatidylinositol linkage on infected cells, formation of a soluble lipophilic hexamer secreted by infected cells, or binding back of the hexameric NS1 to the surface of uninfected cells via glycosaminoglycan interactions (9, 10).

Secreted soluble DENV NS1 (sNS1) is organize in patient serum during acute illness and is used as a diagnostic indicator of acute DENV infection. lofty levels of sNS1 are associated with increased disease severity, although it is unclear whether this measure has functional significance or is just a marker of higher viremia in ascetic cases. sNS1 can bind host complement components directly and inhibit complement activation in solution and on the cell surface, whereas sNS1-antibody complexes can consume complement in the context of secondary human DENV infection (11–14). Although sNS1 binds to endothelial cells (12), the functional consequences of this interaction for disease pathogenesis are poorly understood. Antibodies to NS1 cross-react with platelets, endothelial cells, and thrombin and are postulated to contribute to pathogenesis (15–17). However, vaccination with NS1 or passive transfer of monoclonal antibodies to NS1 (anti-NS1 mAbs) can at least partially protect against DENV infection in mice that are injected intracranially (18–21) or intraperitoneally (22) with DENV, indicating that anti-NS1 antibodies can exist protective and not pathogenic (23). NS1 is considered a promising vaccine candidate (23–26) because it is highly conserved across DENV serotypes and because it should not generate disease-enhancing antibodies.

Although the levels of sNS1 correlate with disease severity, a direct role for NS1 in triggering endothelial disruption has not yet been established; therefore, they evaluated whether NS1 compromises endothelial cell integrity and contributes to vascular leakage in vitro and in vivo, respectively, and whether the immune response to NS1 after vaccination provides protection in a lethal mouse model of DENV vascular leak syndrome.

RESULTS NS1 triggers vascular leak–mediated pathogenesis in vivo

We hypothesized that NS1 might elicit direct pathogenic effects in DENV disease and sought to test its outcome in both the absence and presence of viral replication. For every bit of experiments, they used commercially obtained recombinant NS1 produced in human embryonic kidney (HEK) 293 cells or in S2 cells. These NS1 preparations are highly purified (fig. S1A) and reported to exist hexameric in structure; they confirmed that almost every bit of the proteins are in the oligomeric figure (fig. S1B). The NS1 preparation is reported to exist endotoxin-free, which they confirmed using the Limulus Amebocyte Lysate assay [<0.1 EU/ml in 25 μg of NS1 from DENV1 to DENV4 NS1 (HEK 293) or DENV2 NS1 (S2)]. C57BL/6 mice deficient in the interferon-α/β receptor (Ifnar−/−) inoculated with DENV2 NS1 (10 or 20 mg/kg) in the absence of DENV infection exhibited morbidity on days 2 to 4 post-inoculation (p.i.), although they recovered by day 5 p.i. (fig. S2A). Moreover, 100% of naïve mice inoculated intravenously with DENV2 NS1 (10 mg/kg) combined with a sublethal dose [106 plaque-forming units (PFU)] of DENV2 strain D220 (hereafter referred to as DENV2) succumbed 3 to 4 days p.i., equivalent to mice receiving a lethal dose (107 PFU) of DENV2 (Fig. 1A). In contrast, mice receiving a sublethal dose of DENV2 lonely or ovalbumin (OVA) (10 mg/kg) with a sublethal dose of DENV2 exhibited 100% survival and petite morbidity. Mice injected with less DENV2 NS1 (5 mg/kg) combined with a sublethal dose of DENV2 every bit of exhibited signs of disease starting day 2 p.i., and two of six mice succumbed on day 3 p.i. (Fig. 1A), suggesting a dose-dependent outcome of NS1 in mediating ascetic dengue disease.

Fig. 1. NS1 triggers vascular leak–mediated pathogenesis in vivo.

(A) Ifnar−/− mice were infected intravenously with a lethal dose of DENV2 (107 PFU) lonely (n = 7), a sublethal dose of DENV2 (106 PFU) with DENV2 NS1 [5 mg/kg (n = 6) or 10 mg/kg (n = 7)], a sublethal dose of DENV2 with OVA [5 mg/kg (n = 6) or 10 mg/kg (n = 7)] as a negative control, or DENV2 NS1 (10 mg/kg) lonely (n = 7). A Kaplan-Meier survival curve is shown, with data derived from two independent experiments. Mice given NS1 (10 mg/kg) lonely or OVA (5 or 10 mg/kg) + 106 PFU of DENV2 exhibited statistically significant survival using a nonparametric Mantel-Cox log-rank test (P = 0.022) compared to mice given NS1 (10 mg/kg) + 106 PFU of DENV2. (B to D) Evans blue dye was injected intravenously into mice 3 days after treatment with DENV2 NS1 (10 mg/kg) (n = 7), DENV2 NS1 (20 mg/kg) (n = 4), OVA (10 mg/kg) (n = 6), NS1 (10 mg/kg) + 106 PFU of DENV2 (n = 4), OVA + 106 PFU of DENV2 (n = 4), or 106 (n = 4) or 107 PFU of DENV2 lonely (n = 7), as indicated. The dye was allowed to circulate for 1 h before mice were euthanized, tissues were harvested, and Evans blue was extracted in formamide and quantified in (B) lung, (C) liver, and (D) tiny intestine tissue by measuring absorbance at OD610. In every bit of tissues, the mice given NS1 (20 mg/kg) or NS1 (10 mg/kg) lonely or NS1 (10 mg/kg) + 106 PFU of DENV2 had significantly higher levels of Evans blue than the OVA controls. (E) Ifnar−/− mice were administered DENV2 NS1 (10 mg/kg) or OVA (10 mg/kg) alone, NS1 or OVA (10 mg/kg) plus 106 PFU of DENV2, or 106 or 107 PFU of DENV2 alone, as indicated (n = 4 per group). Plasma was collected 3 days after treatment, and the levels of NS1 were measured by NS1-specific capture enzyme-linked immunosorbent assay (ELISA). (B to E) Data were derived from two independent experiments and were analyzed by nonparametric Mann-Whitney analysis. *P < 0.05; **P < 0.01.

We performed parallel experiments to measure vascular leakage as detected by tissue-associated Evans blue dye, which quantifies leakage out of the circulatory system when injected intravenously. They evaluated the vascular leakage on day 3 p.i. because this day was when they observed the greatest morbidity in the NS1-injected mice (fig. S2A). Mice receiving NS1 (10 mg/kg) plus a sublethal dose of DENV2 or a lethal dose of DENV2 displayed lofty levels of Evans blue dye in the lung, liver, spleen, and tiny and large intestines (Fig. 1, B to D, and fig. S2, B and C), indicating substantial vascular leakage. Mice receiving NS1 (10 or 20 mg/kg) lonely contained significantly higher levels of Evans blue in every bit of tissues than mice administered OVA as a negative control, whereas mice receiving sublethal doses of DENV2 (plus or minus OVA) had lower but measurable levels of Evans blue (Fig. 1, B to D, and fig. S2, B and C). These data demonstrate a role for NS1 lonely in inducing vascular permeability in vivo.

In a part set of mice, they evaluated the levels of circulating NS1, viremia, and selected cytokines in serum 3 days p.i., when maximum morbidity and mortality were observed, with or without NS1 or OVA as above. Injection of NS1 (10 mg/kg) lonely resulted in ~1 μg/ml circulating NS1 in serum at day 3 p.i., only slightly higher than levels in mice infected with a lethal dose of DENV2 (Fig. 1E). Mice infected with a sublethal dose of DENV2 lonely or combined with OVA (10 mg/kg) had ~0.25 μg/ml circulating NS1 at day 3 p.i., whereas mice infected with a sublethal dose of DENV2 and NS1 (10 mg/kg) yielded an medium of ~2 μg/ml circulating NS1 at day 3 p.i. (Fig. 1E). They too evaluated the levels of NS1 every 12 h after inoculation with NS1 (10 mg/kg) and organize that they decreased from ~15 μg/ml at 12 h p.i. to ~1 μg/ml at 72 h p.i. (fig. S2D); these levels are consistent with the ambit seen in humans with ascetic dengue (~1 to >10 μg/ml) (27, 28). Viremia levels were comparable between mice receiving NS1 plus a sublethal dose of DENV2 and mice infected with a lethal dose of DENV2, and both were 10-fold greater than viremia levels in OVA plus a sublethal dose of DENV2 or a sublethal dose of DENV2 lonely (fig. S2E). With respect to cytokines often associated with DHF/DSS, they organize that inoculation of NS1 lonely or addition of NS1 to a sublethal dose of DENV2 significantly increased levels of TNF-α and IL-6 compared to negative controls, to levels similar to those observed in mice experiencing a lethal DENV2 infection (Fig. 2, A and B). In sum, the simultaneous injection of NS1 with a sublethal dose of DENV2 resulted in a lethal infection associated with vascular leakage, and NS1 lonely increased vascular leakage in vivo. Furthermore, injection of NS1 in both the presence or absence of viral infection led to increased levels of faultfinding inflammatory cytokines.

Fig. 2. NS1 causes increased production of inflammatory cytokines in vivo in the presence or absence of DENV2.

Ifnar−/− mice were administered DENV2 NS1 (10 mg/kg) or OVA (10 mg/kg) alone, NS1 or OVA (10 mg/kg) plus 106 PFU of DENV2, or 106 or 107 PFU of DENV2 alone, as indicated (n = 4 per group). (A and B) Plasma was collected 3 days after treatment, and the levels of (A) IL-6 and (B) TNF-α were measured by ELISA. Mice given NS1 (10 mg/kg) + 106 PFU of DENV2 or NS1 (10 mg/kg) lonely had significantly higher levels of both cytokines than controls receiving OVA. Data were derived from two independent experiments and were analyzed by nonparametric Mann-Whitney analysis. *P < 0.05.

NS1 causes endothelial permeability in vitro

To directly evaluate a feasible role for NS1 in triggering endothelial cell dysfunction, NS1 lonely was incubated with cultures of a human pulmonary microvascular endothelial cell (HPMEC) line. HPMECs were grown on Transwell permeable membranes as a model of barrier role in vitro and incubated with DENV2 NS1 (0.2 to 20 μg/ml). Endothelial cell permeability was examined by measuring the transendothelial electrical resistance (TEER) of the HPMECs at different time points after treatment with NS1. TNF-α, known to induce endothelial barrier dysfunction, was used as a positive control and resulted in decreased TEER values (Fig. 3A). DENV2 NS1 induced a significant (P < 0.0001) dose-dependent extend in endothelial permeability compared to TEER baseline values exhibited by untreated controls and treatment with control protein (20 μg/ml OVA), starting ~2 h post-treatment (hpt) and persisting for 12 to 24 hpt depending on the dose (Fig. 3A and fig. S3A). Similar results were observed in primary human umbilical vein endothelial cells (HUVEC; fig. S3B). They then tested NS1 from DENV1, DENV2, DENV3, and DENV4 at 5 or 20 μg/ml on HPMEC monolayers (Fig. 3B and fig. S3C). NS1 from every bit of four serotypes resulted in a significant (P < 0.0001) subside in TEER; DENV1 and DENV2 NS1 caused the greatest reduction, from 3 to 13 hpt, whereas DENV3 and DENV4 NS1 displayed similar decreases from 9 to 13 hpt. In contrast, NS1 from West Nile virus (WNV), a closely related flavivirus that causes encephalitis but no systemic vascular leak, did not trigger reduction in TEER, nor did another DENV protein (E) when administered at the identical concentration (Fig. 3B and fig. S1A). Thus, NS1 lonely from every bit of four DENV serotypes, but not WNV, can directly trigger increased endothelial cell permeability.

Fig. 3. NS1 induces endothelial permeability in human pulmonary endothelial cells in vitro.

Confluent monolayers of HPMECs grown on Transwell inserts were incubated for 48 h with NS1, and TEER (ohm) was measured at indicated time points. Relative TEER (ratio of resistance values between experimental and untreated cells) was plotted. Data were normalized to inserts containing medium only. (A) Increasing concentrations of DENV2 NS1 (0.5 to 5 μg/ml) or TNF-α (1 ng/ml) were added to HPMEC monolayers, and TEER was measured at the indicated time points. (B) DENV1, DENV2, DENV3, DENV4 NS1, WNV NS1, or DENV2 E protein (5 μg/ml) was added to HPMEC monolayers, and TEER was analyzed at indicated time points. OVA (20 μg/ml) was used as a treatment control. Results are representative of three independent experiments using duplicate Transwells. error bars testify SEM. NS1, DENV2 NS1 (5 μg/ml).

NS1 vaccination protects against lethal DENV–induced vascular leak syndrome

Given the competence of NS1 to induce vascular leak, they hypothesized that vaccination with NS1 might constitute a strategy for protecting against ascetic dengue disease. To investigate whether immune responses against NS1 could preclude lethal vascular leak–associated DENV disease, they immunized Ifnar−/− mice three times intraperitoneally with 20 μg of DENV2 NS1 and several different adjuvant systems over a 6-week age (days 1, 14, and 42) and challenged the mice with a lethal DENV2 infection 2 weeks after the eventual immunization (day 56). Mice immunized with DENV2 NS1 and monophosphoryl lipid A (MPLA)/AddaVax were completely protected against a lethal systemic challenge (Fig. 4A) and exhibited petite morbidity. Similarly, NS1/MPLA/AddaVax-immunized mice were protected against antibody-enhanced lethal challenge with DENV2 (29, 30) (Fig. 4A). no one at all of the OVA-immunized mice survived the high-dose or ADE lethal challenge. A low-dose (105 PFU) primary homologous infection with the parental DENV2 strain PL046 provided 100% protection against lethal challenge as a positive control (Fig. 4A). Thus, immune responses to adjuvanted NS1 protein lonely were sufficient to provide complete protection, comparable to immunity induced by primary DENV infection. They then immunized mice with NS1, OVA, or DENV2 PL046, subjected them to lethal DENV2 challenge, and evaluated levels of NS1 in the serum. Sera from NS1-immune mice 3 days after the challenge contained almost no detectable NS1 compared to ~0.75 μg/ml in OVA-immunized mice, whereas NS1 levels in DENV2 PL046-immune mice were reduced to a lesser degree (~0.45 μg/ml) (Fig. 4B). NS1-immune and DENV-immune mice too displayed lower viremia in serum and lower viral load in bone marrow, spleen, and tiny intestine after challenge compared to OVA-immunized mice (Fig. 4, C and D, and fig. S4, A and B). These data suggest that the immune response to NS1 protected against mortality by greatly reducing circulating NS1 as well as decreasing the peripheral viral load.

Fig. 4. NS1 vaccination protects against lethal DENV–induced vascular-leak syndrome.

(A) Mice were immunized intraperitoneally with 20 μg of DENV2 NS1 (n = 12) or OVA (n = 8) combined with MPLA/AddaVax adjuvants on days 0, 14, and 42 or infected with a sublethal dose (105 PFU) of DENV2 PL046 (n = 12) at day 0; on day 56, half were challenged intravenously with a lethal dose of 107 PFU of DENV2 and half with lethal antibody-enhanced DENV2 infection. A Kaplan-Meier survival curve is shown. Mice immunized with NS1 were significantly protected compared to OVA controls from both ADE (P = 0.003) and high-dose (hi-dose) (P = 0.001) lethal challenge. (B) NS1 levels in serum were measured by NS1-specific capture ELISA in NS1-immunized (n = 6) or OVA-immunized (n = 6) mice and DENV2 PL046–immune (n = 3) mice 3 days after DENV2 lethal challenge. Circulating NS1 was significantly higher in OVA-immunized compared to NS1-immunized mice after challenge; *P < 0.05; **P < 0.01. (C and D) The viral RNA copy number was measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR) in (C) serum and (D) bone marrow 3 days post-lethal challenge. OVA-immunized mice (n = 6) had higher levels of viral RNA in both tissues compared to NS1-immunized mice (n = 6); *P < 0.05; **P < 0.01. The restrict of detection is indicated by a dashed line. GAPDH, glyceraldehyde phosphate dehydrogenase. GE, genome equivalent. (E) Mice were immunized with 20 μg of NS1 from DENV1 (n = 8), DENV2 (n = 8), DENV3 (n = 5), or DENV4 (n = 5) or OVA (n = 10) with MPLA/AddaVax adjuvants as above, or 105 PFU of DENV2 PL046 (n = 10) at day 0 and then challenged intravenously on day 56 with a lethal dose of DENV2. Mice immunized with the four DENV NS1 serotypes separately were significantly protected against lethal challenge compared to OVA-immunized groups (DENV1, P = 0.002; DENV2, P = 0.008; DENV3, P = 0.004; DENV4, P = 0.014). Data were derived from three independent experiments.

Because relatively lofty homology exists in the amino acid sequence of NS1 within and across the four DENV serotypes (31), they examined whether immunization with NS1 could protect against a heterologous, lethal DENV infection. Mice were immunized as above with DENV1, DENV2, DENV3, or DENV4 NS1, challenged 2 weeks after the third immunization with a lethal dose of DENV2, and monitored for morbidity and mortality. As expected, DENV2 NS1 provided 100% protection against lethal homologous DENV2 challenge (Fig. 4E). Immunization with DENV1 NS1 provided 75% protection, and DENV3 or DENV4 NS1 provided 60% protection against DENV2 lethal challenge (Fig. 4E), demonstrating sufficient immune cross-reactivity to NS1 epitopes to provide partial protection across serotypes. Serum was obtained from mice 1 week after the third immunization with NS1 or OVA, which coincided with 7 weeks after DENV2 PL046 primary infection. They tested the prechallenge immune serum by ELISA for reactivity to NS1 of every bit of four DENV serotypes (fig. S5). DENV NS1-immune sera bound strongly to NS1 from the homologous serotype and too exhibited substantial cross-reactivity to NS1 from the other three serotypes. In addition, the plane of anti-NS1 antibody binding and serotype cross-reactivity was greater in NS1-immunized mice than in natural infection with the different DENV serotypes (fig. S5).

NS1-immune serum and anti-NS1 mAbs shroud NS1-induced vascular leak

To determine whether antibodies from NS1-immunized mice could preclude the direct outcome of NS1 on endothelial permeability, they tested NS1- or OVA-immune sera from vaccinated mice on HPMEC monolayers using the TEER assay. Intact NS1-immune sera inhibited the NS1-induced subside in TEER, whereas OVA-immune sera provided no inhibition (Fig. 5A). Heat-inactivated NS1-immune sera too inhibited the NS1-induced subside in TEER, demonstrating that the inhibition was not dependent on complement. Sera from DENV2 PL046-immune mice partially inhibited the reduction in TEER, consistent with the lower plane of anti-NS1 antibodies observed in DENV2-immune sera compared to the plane in NS1-immune sera (fig. S5). To further verify the specificity of the antibody-mediated blocking of NS1 role in vitro, a panel of anti-NS1 mAbs was evaluated, and several anti-NS1 mAbs (for example, 2B7 and 1H7.4) were shown to inhibit the NS1-induced subside in TEER (Fig. 5B). Several other anti-NS1 mAbs (for example, 2E9.E2) did not inhibit the reduction in TEER (Fig. 5B), demonstrating that blocking inevitable but not every bit of epitopes leads to the loss of NS1 disruption of endothelial integrity. As expected, mAbs directed to another DENV protein, E (for example, 3H5), did not shroud NS1 role in the TEER assay, nor did the anti-NS1 mAbs lonely occupy any outcome on endothelial permeability (Fig. 5B).

Fig. 5. NS1-immune serum and anti-NS1 mAbs shroud NS1-induced endothelial permeability in vitro.

(A) Monolayers of HPMECs grown on Transwell inserts were incubated for 48 h with both NS1 (5 μg/ml) and anti-NS1 serum (1:10 dilution), NS1 alone, or anti-NS1 serum alone, and TEER (ohm) was measured at indicated time points. OVA (5 μg/ml) was used as a protein control. Anti-OVA and anti-DENV2 PL046 sera were too included as controls. Results are representative of two independent experiments with duplicate Transwells. (B) Monolayers of HPMECs grown on Transwell inserts were incubated for 48 h with both NS1 (5 μg/ml) and NS1-specific mAbs (10 μg/ml) or either NS1 or anti-NS1 mAbs alone, and TEER (ohm) was measured at indicated time points. A mAb specific to DENV E protein (3H5) was used as an isotype control. Results are representative of three experiments, each with duplicate Transwells. error bars testify SEM.

To investigate the role of anti-NS1 antibodies in the protective immunity observed against NS1 toxicity in vivo, they passively transferred sera from NS1- or OVA-immunized mice (both in combination with MPLA/AddaVax) concurrently with a lethal challenge of NS1 plus a sublethal dose of DENV2. The passive transfer of 300 μl of anti–NS1-immune serum provided 100% protection from lethality, whereas mice receiving 300 μl of anti-OVA sera succumbed (Fig. 6A). Therefore, antibodies from NS1-vaccinated mice were sufficient to inhibit NS1-mediated toxicity, suggesting that anti-NS1 antibodies provide a faultfinding component of immune protection against lethal DENV2 challenge observed in NS1-vaccinated mice.

Fig. 6. NS1-immune serum and anti-NS1 mAb shroud NS1-induced lethality in vivo.

(A) Pooled serum (300 μl) from NS1-immunized (n = 7), OVA-immunized (n = 7), or DENV2 PL046–immune (n = 4) mice was transferred intraperitoneally to naïve Ifnar−/− mice before intravenous injection of NS1 (10 mg/kg) + 106 PFU of DENV2. Anti-NS1 or anti-OVA serum was transferred to naïve mice before injection of OVA (10 mg/kg) + 106 PFU of DENV2 (n = 4 per group) as controls (ctrl). Mice were followed for survival for 14 days p.i. Mice administered NS1-immune serum followed by NS1 + 106 PFU of DENV2 were significantly protected (P = 0.014) against lethality compared to mice given OVA-immune serum (n = 7), using the log-rank Mantel-Cox test. Data were derived from two independent experiments. (B) Ifnar−/− mice were infected intravenously with 107 PFU of DENV2 lonely (n = 3), 106 PFU of DENV2 lonely (n = 3), 106 PFU of DENV2 with DENV2 NS1 (10 mg/kg), or 106 PFU of DENV2 with OVA (10 mg/kg), as indicated. Mice injected with DENV2 combined with DENV2 NS1 or OVA were concomitantly injected intraperitoneally with 200 μg of isotype control mAb or anti-NS1 mAb (1H7.4) (n = 4 for every bit of groups). Kaplan-Meier survival curves are shown, where mice were monitored for morbidity and survival for 10 days p.i. Mice given anti-NS1 mAbs were significantly protected (P = 0.0069) against lethality compared to mice given the isotype control mAbs by log-rank Mantel-Cox test.

Finally, they tested the competence of an anti-NS1 mAb that blocked TEER role in vitro (Fig. 5B) to protect against NS1-induced toxicity in vivo. The anti-NS1 mAb was administered to mice immediately after inoculation with a sublethal viral dose plus NS1 (10 mg/kg), and the mice were monitored for morbidity and mortality for 10 days. Mice receiving the lethal virus inoculum as a positive control and the mice receiving a sublethal viral dose plus NS1 (10 mg/kg) and the isotype control succumbed to the vascular leak disease; however, the mice receiving the sublethal viral dose plus NS1 (10 mg/kg) and the anti-NS1 mAb demonstrated 100% survival (Fig. 6B). Therefore, both polyclonal and monoclonal anti-NS1 antibodies can inhibit lethal vascular permeability in vivo and can shroud the competence of NS1 to trigger endothelial cell dysfunction in vitro.

DISCUSSION

We occupy established that DENV NS1 causes vascular leak in vivo and induces increased permeability of human endothelial cells in vitro, both of which can exist specifically blocked with NS1-immune polyclonal mouse sera or anti-NS1 mAbs. These results suggest that NS1 can occasions endothelial dysfunction leading to vascular leakage during ascetic dengue disease. In addition, they demonstrated that immune responses elicited by vaccination with DENV2 NS1 protect against lethal challenge with high-dose or antibody-enhanced DENV2 infection, comparable to immunization with a primary sublethal DENV2 infection. Furthermore, vaccination with DENV1, DENV3, or DENV4 NS1 provided substantial protection against a heterologous DENV2 lethal challenge. Their findings, along with those in the accompanying paper by Modhiran et al. (32), add an notable and previously overlooked component to the induction of vascular leak in ascetic dengue.

NS1 levels occupy been shown to correlate with disease severity (11, 28), and although NS1 antigenemia peaks concurrently with viremia early in disease, cumulative effects of NS1 on the endothelium might occur after several days of antigenemia, during the “critical phase” on days 4 to 6 post-symptom onset when hypovolemic shock is observed. This would exist consistent with their finding that mice displayed the greatest vascular leak and morbidity 3 days after injection with NS1 lonely and experienced the most ascetic disease 3 days after inoculation with NS1 combined with a sublethal dose of DENV. They organize evidence of vascular leak in the lung, liver, spleen, and lymph nodes of mice, which parallels the finding that most of the histopathological damage identified in deadly human cases of dengue disease occurs in these tissues as well (33, 34). In terms of the physiological relevance of their findings, the in vitro and in vivo effects of NS1 occurred at concentrations similar to those reported in patients with DHF/DSS (27, 28). They too observed a correlation between the amount of circulating NS1, the degree of vascular leak, and production of inflammatory cytokines (IL-6 and TNF-α) in vivo. This is consistent with NS1 triggering increased production of vasoactive cytokines by monocytes and macrophages, as shown in the accompanying paper (32). Finally, they studied the effects of NS1 on human endothelial cell monolayers (HPMECs and HUVECs) and demonstrated that DENV, but not WNV, NS1 can disrupt endothelial cell permeability in a dose- and time-dependent manner. These in vitro results testify that NS1 can act directly on the endothelial barrier to extend vascular permeability. These findings are consistent with those of Modhiran et al. (32), which showed that NS1 triggers endothelial permeability directly in human dermal microvascular endothelial cells within several hours of addition. Several feasible mechanisms could account for the direct contribution of NS1 to vascular leak, including disruption of components of the endothelial glycocalyx layer and extracellular matrix and mislocalization or degradation of intercellular-junction proteins triggered by activation of intracellular signaling pathways (35, 36).

We note here that vaccination with NS1 protects against homologous and heterologous lethal challenge in Ifnar−/− mice, currently the in vivo model that best mimics features of ascetic human dengue (30). Previous studies using intracranial challenge demonstrated that immunization with DENV2 NS1 protected against homologous but not heterologous DENV serotypes (18). Earlier labor too demonstrated that the passive transfer of NS1-specific mouse polyclonal antibodies or mAbs protected against intracranial homologous challenge with DENV2 (20, 21). More recent labor has shown that an NS1 DNA vaccine as well as NS1 combined with a heat-labile toxin adjuvant can protect against intracranial challenge (23, 25, 26) in BALB/c mice. Their data note that vaccination with NS1 protects against lethal DENV–induced vascular leakage syndrome in mice and that anti–NS1-immune sera or anti-NS1 mAbs can preclude NS1-mediated vascular leakage both in vivo and in vitro. The antibody-derived immunity to NS1 could exist mediated via an Fc-dependent pathway, complement-dependent pathways (37), or inhibition of the pathogenic effects of NS1. They suggest that antibodies to NS1 generated from vaccination can extend virus clearance as well as counteract the vasoactive effects of NS1. Together, their data testify that immunization with NS1 could provide faultfinding protection against ascetic DENV disease without the risk of ADE and wrangle for the inclusion of DENV NS1 in dengue vaccines.

Although anti-NS1 antibodies can occupy a potential pathogenic outcome because of cross-reactivity with human endothelial cells, platelets, or thrombin (15–17), they observed a protective rather than a pathogenic outcome of anti-NS1 polyclonal antibodies or mAbs on endothelial cells. Heat-inactivated anti-NS1 sera lacking lively complement components blocked NS1-mediated endothelial permeability, as did anti-NS1 mAbs added to HPMECs in the absence of complement. Their results demonstrate that DENV NS1 has a direct outcome on endothelial cells and suggest that NS1 triggers vascular leakage in the absence of antibodies or complement. Finally, although antibodies to NS1 are reported more frequently after secondary DENV infection (38), it is feasible that the repertoire of anti-NS1 antibodies and the amount of free NS1 (27, 28) may disagree in patients who develop ascetic as compared to mild disease, a topic of future investigation.

In addition, they organize that exogenous NS1 resulted in increased viral tribulation in vivo, which may too exacerbate disease. This is consistent with data showing increased viral output in DENV-infected cells exposed to exogenous NS1 (39). It too could result from the competence of NS1 to antagonize activation of complement pathways (8, 13), which can directly counteract DENV in plasma (13, 14, 40) and control virus replication in vivo. They too eminent that mice receiving a lethal dose of DENV2 lonely displayed lofty levels of vascular leakage and inflammatory cytokines with only a partial extend in circulating NS1. This suggests that the extend in vascular leakage leading to mortality in the high-dose infection with DENV2 is partially attributable to virus-induced damage in addition to the pathogenic effects of NS1. This is consistent with a report by Watanabe et al. (41), which demonstrated differences among DENV2 strains in relation to mortality that were independent of the NS1 levels in a mouse model of DENV infection. It is too feasible that strain-related differences in NS1 can modulate the pathogenic properties of NS1.

One of the limitations of this study was their competence to explore different kinetics of NS1 delivery in vivo. They delivered the NS1 in vivo in a solitary dose, which resulted in circulating levels initially 15 μg/ml at 12 h (on the lofty discontinuance of what has been seen in humans) that subside to ~1 μg/ml at 72 h (more often the ambit seen in ascetic dengue cases); the rapid decline in NS1 levels may exist attributable to protein degradation or clearance from the bloodstream. However, another paper that examined levels of NS1 in a mouse model of DENV2 infection reported circulating levels of NS1 in mice of 8 to 30 μg/ml (41). It will exist tantalizing to explore different kinetics of delivery of NS1 in vivo. This study was too limited by their competence to differentiate, in the mouse model, between NS1 triggering endothelial permeability directly versus NS1 triggering mediators that may exist acting on endothelial cells. Evaluating the precise role of signaling molecules and inflammatory mediators, including cytokines, in the mechanisms of NS1-induced vascular leak in vivo requires study in genetically deficient mice and the employ of various inhibitors and/or antibodies; these studies are currently underway. In part in vitro experiments, they occupy identified cytokine-independent, endothelial cell–intrinsic mechanisms through which NS1 leads to increased endothelial permeability, in addition to the cytokine-dependent pathways triggered by NS1 described by Modhiran et al. (32).

To date, the paramount theory to account for dengue shock invokes immunopathogenic processes thought to lead to endothelial dysfunction; here, they submit that DENV NS1 protein by itself is a key component in triggering the ascetic vascular leakage associated with life-threatening dengue. This labor opens the door to evaluating potential drug therapeutics that can inhibit NS1-mediated disease. Likewise, their NS1 vaccination data showing protection against DENV lethal vascular leak syndrome points to the significance of including NS1 in dengue vaccine formulations.

MATERIALS AND METHODS Study design

We designed these studies to note that DENV NS1 plays a role in causing vascular leakage and that immunization with NS1 could preclude DENV-induced systemic disease. They first showed that recombinant NS1 as a vaccine could protect against lethal DENV–induced vascular leak syndrome and then followed this with studies of NS1 lonely or NS1 combined with a sublethal DENV infection to characterize disease due to increased levels of NS1. Mice infected with DENV with and without NS1 were evaluated on a morbidity scale from 1 to 5 (30). Mice were monitored every 12 h after infection, followed every 6 h when displaying a score of >3, and euthanized immediately when they became moribund (score of 5). NS1 immunization experiments included negative controls consisting of OVA-immunized or unimmunized mice that were not protected and DENV2 PL046-immunized mice as positive controls that were protected against lethal challenge. In NS1 vascular leak experiments, OVA-injected mice were used as negative controls in comparison to NS1-injected mice, and sublethal DENV infection plus OVA was included as a negative control compared to sublethal DENV infection plus NS1. Mouse experiments testing mortality or protection from lethal DENV challenge had endpoints 10 days after infection, and no NS1-immunized, NS1-injected, or DENV-infected mice were excluded from the study. Mouse experiments were performed in duplicate, with numbers totaling four or greater in each group. They then hypothesized that DENV NS1 directly induces endothelial permeability and used in vitro TEER experiments with cultured human endothelial cell lines to evaluate this effect. Each TEER experiment was performed two to three times, with each condition including two replicate wells that were each read twice. Each experiment contained the following controls: (i) Transwell inserts containing only medium to determine momentary resistance values of the permeable membrane, (ii) Transwell inserts with cultured human endothelial cells to obtain resistance values of the endothelial cell monolayer alone, and (iii) a positive control consisting of vasoactive molecules such as TNF-α, which is known to extend endothelial permeability. In addition, as an irrelevant protein control, OVA was included in each experiment. For the protection experiments using sera obtained from NS1-vaccinated mice or anti-NS1 mAbs, Transwells containing only sera or anti-NS1 mAbs were included to exclude any outcome on endothelial permeability induced by these components. TEER values were collected every 2 h for 12 h and at 24 h, then the medium was replaced to reestablish the integrity of the monolayer, and the TEER values were read at 26 and 48 h. No blinding or randomization was performed for either the mouse studies or the TEER experiments.

Mice

Six- to eight-week veteran Ifnar−/− C57BL/6 mice were bred and maintained under specific pathogen–free conditions at the University of California, Berkeley, Animal Facility. Experiments were performed strictly following guidelines of the American Veterinary Medical Association and the steer for the care and employ of Laboratory Animals of the National Institutes of Health. The Animal care and employ Committee of the University of California, Berkeley, approved every bit of experiments (protocol R252-1012B).

Cell cultures and viruses

All viruses were propagated in the Aedes albopictus C6/36 cell line [American ilk Culture Collection (ATCC)] and titered by plaque assay on baby hamster kidney cells (BHK21, clone 15). DENV2 PL046 was obtained originally from H.-Y. Lei (National Cheng Kung University, Taiwan). DENV2 D220 was produced in their laboratory from the parental strain DENV2 PL046 (42). An HPMEC line (HPMEC.ST1.6R) was donated by J. C. Kirkpatrick (Institute of Pathology, Johannes Gutenberg University, Germany), propagated (passages 5 to 8), and maintained at 37°C in humidified air with 5% CO2 in endothelial cell basal medium-2 supplemented with growth factors, antibiotics, and fetal bovine serum as per the manufacturer’s specifications (Clonetics, Lonza). HUVECs (passage 3) were donated by M. Lodoen (University of California, Irvine) and maintained as described above.

Recombinant NS1 proteins and anti-NS1 mAbs

Recombinant NS1 proteins from DENV1, DENV2, DENV3, and DENV4 and WNV, greater than 95% purity and certified to exist free of endotoxin contaminants, were produced by indigenous Antigen in HEK 293 cells; recombinant DENV2 NS1 produced in S2 cells was obtained from Merck. The recombinant DENV2 envelope (recE), expressed in a baculovirus expression vector system (GenScript) using lofty Five insect cells, was purified by an immune-affinity protein G column coupled to 4G2 (anti-E mAb). The NS1 and recE working stocks were too tested using the Endpoint Chromogenic Limulus Amebocyte Lysate (LAL) QCL-1000TM kit (Lonza) and confirmed to exist bacterial endotoxin-free (<0.1 EU/ml per 25 μg of protein). Anti-NS1 mAbs were obtained as follows: 2B7 and 2E9.E2 were generated in the Harris laboratory (P.R.B. and E.H., unpublished), and 1H7.4 was a gift from P. puerile (University of Queensland, Brisbane, Australia). mAb 3H5 (anti-DENV2 E) was obtained from ATCC. Hybridomas were propagated in CELLine culture flasks (Sigma); supernatants were collected, and the mAbs were affinity-purified using a Protein G Sepharose column. The mAbs were eluted from the column, concentrated, sterile-filtered, and titered before use.

Mouse experiments of NS1-induced vascular leak and NS1 vaccination

For studies of NS1-induced vascular leak, Ifnar−/− mice were injected intravenously with NS1 (5 to 20 μg/kg) or OVA protein (10 μg/kg) lonely or NS1 (5 to 10 μg/kg) or OVA (10 μg/kg) in combination with a sublethal dose of 106 PFU of DENV2 D220. part groups of mice were administered 106 PFU of DENV2 D220 lonely as a sublethal control or 107 PFU of DENV2 D220 as a lethal control. Mice were observed every 12 h for morbidity using a scoring system on a scale of 1 to 5 as follows: 1 = healthy; 2 = ruffled fur and mild signs of lethargy; 3 = hunched posture, ruffled fur, and intermediate plane of lassitude and failure to groom; 4 = very lethargic, limited mobility, ruffled fur, and hunched posture; and 5 = moribund with limited to no mobility and inability to achieve food or water. Mice were euthanized immediately when they became moribund. For vascular leak studies and measurement of cytokines and levels of sNS1 and viral RNA, mice were euthanized 72 h after injection, blood was collected via cardiac puncture, and tissues were harvested. For mortality studies, mice were monitored for 10 days after injection. For vaccination studies, Ifnar−/− mice were injected intraperitoneally three times (days 0, 14, and 42) with 20 μg of NS1 or 20 μg of OVA with 1 μg of MPLA (InvivoGen) and AddaVax (0.5% sorbitan trioleate, 5% squalene, and 0.5% Tween 80 in 10 mM sodium citrate buffer; InvivoGen). Two weeks after the third immunization (day 56), mice were challenged with 107 PFU of DENV2 D220 intravenously or 5 μg of 4G2 (anti-E mAb) 24 h before 105 PFU of D220 (ADE). To test the effects of the anti–NS1-immune serum on NS1-induced vascular leak, Ifnar−/− mice were injected with 10 μg of NS1 or 10 μg of OVA in combination with 106 PFU of DENV2 D220, immediately administered 300 μl of polyclonal anti-NS1, anti-OVA, or anti–PL046-immune serum intraperitoneally, and then followed for morbidity and mortality for 10 days. The immune serum used for passive transfer was collected from NS1- or OVA-immunized mice or DENV PL046–infected mice 6 weeks after initial immunization or infection. To test the effects of anti-NS1 mAb on NS1-induced vascular leak, Ifnar−/− mice were injected with 10 μg of NS1 or 10 μg of OVA in combination with 106 PFU of DENV2 D220, immediately given 200 μg of anti-NS1 mAb (1H7.4) or isotype control antibody intraperitoneally, and then followed for morbidity and mortality for 10 days. part groups of mice were given 106 PFU of DENV2 D220 lonely as a sublethal control or 107 PFU of DENV2 D220 as a lethal control.

Enzyme-linked immunosorbent assays

Serum was collected 3 days after the intravenous injection of NS1 with or without DENV infection. Serum was analyzed by NS1-specific capture ELISA using mAb 7E11 (5 μg/ml; a gift from R. Putnak, Walter Reed Army Institute of Research) as a coating antibody, 50 μl of a 1:100 dilution of each mouse serum sample, followed by detection using 100 μl of a biotinylated NS1-specific mAb, 2B7 (4 μg/ml) (P.R.B. and E.H., unpublished). A standard curve of NS1 (7 to 2000 ng/ml) was sprint in parallel to quantify NS1 concentration in every bit of samples. Absorbance [optical density at 405 nm (OD405)] was measured using an Applied Biosystems 7200 microplate reader. To determine levels of NS1-specific antibodies in immunized mice before challenge, serum was obtained 1 week after the third immunization via submandibular bleed. For the ELISA, 50 μl of NS1 (0.5 μg/ml) was coated onto a Nunc Immulon polystyrene plate; after blocking with 5% nonfat parch milk in phosphate-buffered saline (PBS) + 0.05% Tween 20 (PBS-T), 50 μl of a 1:100 dilution of each test serum was added and incubated for 24 h. After washing with PBS-T, biotinylated goat anti-mouse immunoglobulin G (Jackson ImmunoResearch) was added, followed by washing and addition of streptavidin–alkaline phosphatase (Life Technologies) and para-Nitrophenylphosphate (Sigma-Aldrich) substrate, which was read at OD405 as above. Capture ELISAs were performed to quantitate the levels of TNF-α or IL-6 in citrated plasma. Mouse TNF-α and IL-6 ELISA Ready-SET-Go! kits (eBioscience) were used according to the manufacturer’s instructions. The OD405 of each sample was measured, and cytokine levels in serum were expressed in picograms per milliliter.

Quantitation of virus by qRT-PCR

Samples of every bit of tissues were stored in RNA later (Ambion), and RNA was extracted using an RNeasy Mini kit (Qiagen). Serum was generated from entire blood by centrifugation, and RNA was extracted using a QIAamp Viral Recovery RNA kit (Qiagen). Serum viremia levels and tissue viral load were measured by qRT-PCR as described previously (29). Viral load was expressed as either glyceraldehyde phosphate dehydrogenase in genome equivalents per microgram (tissue) or genome equivalents per millilter (serum).

Quantitation of Evans blue vascular leakage

Vascular leakage was quantified by Evans blue dye as previously described (42). Briefly, 200 μl of 0.5% Evans blue dye was injected intravenously 3 days after treatment and allowed to circulate for 1 h before mice were euthanized and cardiac puncture was performed. Tissues were collected in preweighed tubes containing 1 ml of formamide and incubated at 37°C and 5% CO2 for 24 h. Evans blue concentration in extracts was quantified by measuring OD610 in samples and comparing to a standard curve. Data were expressed as nanograms of Evans blue dye per milligram of tissue weight.

Transendothelial electrical resistance

To evaluate the outcome of sNS1 on the integrity of the endothelium, HPMEC or HUVEC monolayers grown on a 24-well Transwell polycarbonate membrane system (Transwell permeable support, 0.4 μM, 6.5-mm insert; Corning Inc.) were incubated with NS1 (0.2 to 20 μg/ml) or OVA (1 to 20 μg/ml) as a negative control. Endothelial permeability was evaluated by measuring TEER in ohms at sequential 2-h time points after the addition of test proteins. TEER was measured using an epithelial voltohmmeter with “chopstick” electrodes (World Precision Instruments). Transwell inserts containing untreated HPMEC cells were used as a negative control, and inserts with medium lonely were used for blank resistance measurements. Relative TEER was expressed as the ratio of resistance value as follows: [ohm (experimental condition) − ohm (medium alone)]/[ohm (nontreated endothelial cells) − ohm (medium alone)]. After 24 h of treatment, 50% of upper and lower chamber media was replaced by fresh endothelial cell medium.

Statistical analysis

Statistical analysis was performed using GraphPad Prism 6 software, and every bit of graphs were generated using Prism 6. Comparison between ELISA and qRT-PCR titers was conducted using a nonparametric Mann-Whitney test. Comparison of survival rates was conducted using a nonparametric log-rank (Mantel-Cox) test and graphed as Kaplan-Meier survival curves. For TEER experiments, statistical significance was determined using a two-way analysis of variance (ANOVA), and differences in treatment were considered significant for P values <0.05.

SUPPLEMENTARY MATERIALS

www.sciencetranslationalmedicine.org/cgi/content/full/7/304/304ra141/DC1

Fig. S1. Recombinant NS1 from DENV1, DENV2, DENV3, DENV4, and WNV is highly purified and oligomeric.

Fig. S2. Morbidity and vascular leakage are induced by NS1.

Fig. S3. NS1 increases endothelial cell permeability in two different endothelial cell lines, HUVEC and HPMEC.

Fig. S4. Viremia after lethal DENV challenge is reduced by immunization with DENV2 NS1.

Fig. S5. Immunization with DENV NS1 and DENV infection induces serotype cross-reactive anti-NS1 antibodies.

REFERENCES AND NOTES
  • Dengue Haemorrhagic Fever: Diagnosis, Treatment, Prevention and Control (World Health Organization, Geneva, ed. 2, 1997), pp. 12–23.

  • Acknowledgments: They thank S. Zompi, S. Orozco, and S. Balsitis for early contribution to the project; M. Diamond, S. Halstead, J. Coloma, and A. Green for helpful discussions and advice; M. Schmid and M. Diamond for useful comments on the manuscript; B.-A. Coller for scientific discussion and enabling purchase of NS1; A. Azliyati for facilitating transfer of HPMECs; P. puerile for the mAb 1H7.4; and G. Firestone for loaning necessary equipment. Funding: This labor was supported by grant U54 AI65359 (E.H.) from the U.S. National Institute of Allergy and Infectious Diseases. Author contributions: P.R.B., H.P.-G., and E.H. conceived and designed the experiments. S.S.K. and K.H. performed the in vivo experiments and ELISAs; H.P.-G. and D.R.G. performed the TEER experiments, Western blots, and silver-stained gels. P.R.B., H.P.-G., S.S.K., D.R.G., K.H., and E.H. analyzed the data. P.R.B., H.P.-G., S.S.K., D.R.G., and E.H. wrote the paper. Competing interests: The authors declare that they occupy no competing monetary interests. Data and materials availability: Anti-NS1 mAbs are available upon request.

  • Interventions To preclude Retinopathy of Prematurity: A Meta-analysis | killexams.com real questions and Pass4sure dumps

    Interventions To preclude Retinopathy of Prematurity: A Meta-analysis | Review Articles | Pediatrics

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    Elevation of ω-3 Polyunsaturated Fatty Acids Attenuates PTEN-deficiency Induced Endometrial Cancer development through Regulation of COX-2 and PGE | killexams.com real questions and Pass4sure dumps

    Animals

    All experimental protocols were approved by the Research Ethics Committee of the Nanjing Medical University. every bit of experiments were conducted in compliance with the guidelines for the care and employ of laboratory animals and approved by Institutional Animal care and employ Committee of Nanjing Medical University. The unvarying diet was from Xietong Biotechnology Co.Ltd (Jiangsu, China). The fish oil was from ShangHai HOPE industry Co. Ltd (Shanghai, China). The lofty ω-3 PUFAs diet was made by the unvarying diet mixed with 5% Fish-Oil (FO). The animals were fed food and water ad libitum, housed at 22 °C and a 12-h light-dark cycle.

    Materials

    All cell culture reagents were purchased from Gibco BRL Technology. AA, EPA, DHA, propidium iodide (PI), and PUFA analytical standards were obtained from Sigma-Aldrich (St. Louis, MO, USA). Water-soluble tetrazolium (WST) reagent was from Dojindo Laboratories (Kumamoto, Japan). The following were commercially obtained antibodies: PTEN antibody, Ki-67 antibody, Cyclin D1 antibody, anti-phospho-Akt antibody (Ser473), Akt antibody (Cell Signaling Technology Danvers, MA, USA); anti-β-actin antibody (Abcam, Cambridge, UK); anti- COX-2 antibody and PGE2 (Cayman Chemical Co, Ann Arbor, MI, USA)

    Generation of mice with mfat-1;PTEN+/−

    The PTENloxP/loxP mice21 and zona pellucida 3 (Zp3)-Cre transgenic mice were obtained from the Model Animal Research heart of Nanjing University (Nanjing, China). The mfat-1 transgenic mice were described in their previous studies20. every bit of above strains of mice occupy a C57BL/6J genetic background. The PTEN gene was deleted specifically from oocytes of primary follicles by crossing Zp3-Cre mice with PTENloxP/loxP mice as previously described53. The female offspring was crossed with manlike mfat-1 transgenic mice to generate four different genotypes for experiments (PTEN+/+, mfat-1, PTEN+/−, mfat-1;PTEN+/−). The PTEN+/+ and mfat-1 mice were served as littermate control.

    The uterus sections from the female mice at 26 weeks of age were subjected to histopathological analyses. unvarying (N): the endometrium is covered with columnar lining epithelium with widely separated tubular glands embedded in a cellular stroma; Simple hyperplasia (SH): increased number of simple tubular glands or the glands covered by crowded hyperplastic epithelium; knotty atypical hyperplasia (CAH): enlarged and irregularly branching endometrial glands; Carcinoma (CA): glands with multilayer cells and extensive cribriform structures or sheets of large pleomorphic epithelial cells.

    PCR Analysis of PTEN and mfat-1 Genotypes

    Tail genomic DNA samples were isolated and amplified by PCR following by a previously described protocol20. Sense primer (5′-CTCCTCTACTCCATTCTTCCC-3′) and anti-sense primer (5′-ACTCCCACCAATGAACAAAC-3′) were used to detect the PTENloxp/loxp allele, sense primer (5′-GTCACCAGGATGCTTCTGAC-3′) and anti-sense primer (5′-TGGCAAGCTTTCAACTTGTTT-3′) were used to detect PTEN+/−, sense primer (5′-GGACCTGGTGAAGAGCATCCG-3′) and anti-sense primer (5′-GCCGTCGCAGAAGCCAAAC-3′) were used to detect the mfat-1, and sense primer (5′-CAGATGAGGTTTGAGGCCACAG-3′) and anti-sense primer (5′-CAGGTGTTATAAGCAATCCC-3′) were used to detect Zp3-Cre transgene. Amplified fragments of 325 bp, 750 bp, 438 bp, and ~500 bp were obtained, respectively.

    Tumor xenograft models

    Four-week-old female BALB/c nude mice were injected with 5 × 106/0.1 ml of RL95-2 cells into the flanks. The mice were randomized into three groups (n = 10). Group 1 was implanted with RL95-2-GFP cells and fed with unvarying diet. Group 2 was implanted with RL95-2-GFP cells and fed with lofty ω-3 PUFAs diet (normal diet mixed with 5% FO). Group 3 was implanted with RL95-2-mfat-1 cells and fed with unvarying diet. Bi-dimensional tumor measurements were taken every two days. Tumor volume was measured along two major axes using calipers. Tumor volume (mm3) was calculated as follows: V = 1/2 LW2 (L: length, W: width).

    Cell culture and proliferation assays

    The endometrial cancer cell lines RL95-2 was obtained from American ilk Culture Collection (ATCC). RL95-2 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) with 5% fetal calf serum, 100 IU/ml penicillin and 100 μg/ml streptomycin at 37 °C with 5% CO2. Cell proliferation assays were performed in 96-well plate. PUFAs were added with serial concentrations (20–100 μM) for 24 h. Then cells were stained with WST at 37 °C for 1 h and quantified by the absorbance at 450 nm.

    Overexpression of mfat-1 by lentiviral transfection

    The description of mfat-1 cDNA has been described previously20. The mfat-1 cDNA was synthesized and cloned into the PLJM1 lentivirus vector (Addgene, Palo Alto, CA, USA). To bear the virus, pMD2.G and psPAX2 were co-transfected with PLJM1-mfat-1 or the vehicle plasmid into 293T cells using X-tremeGENE HP DNA transfection reagent, according to manufacturer’s instructions. To obtain a stable mfat-1 overexpressing cell line, lentivirus-containing supernatant was harvested 48 hours after transfection and used to infect RL95-2 cells.

    Quantitative analysis of gene expression levels in cells and tissues

    Total RNA was prepared using TRIZOL reagent, treated with DNase I, and reverse transcribed with a Superscript kit. Real-time PCR assay was carried out on an Applied Biosystems StepOnePlus™ Systems with the following cycles: 50 °C for 2 min, one cycle; 95 °C for 30 s, one cycle; and 95 °C for 15 s, 58 °C for 30 s and 72 °C for 30 s, 40 cycles.

    Western blotting

    Cells were treated with various PUFA or PGE2 agents at 37 °C for various times, as indicated in the experiments. The cells were collected and subjected to Western blotting analysis. The immunoreactivity was detected by using a standard enhanced-chemiluminescent reaction and analyzed using Image Lab 4.0 analysis software from Bio-Rad.

    Immunohistochemical staining

    The tissue specimens were incubated with antibodies against phospho-Akt, Ki-67, Cyclin D1, and COX-2. The specific signals were detected using EnVision polymer technology, and visualization was performed with 3, 3N-diaminobenzidine tetrahydrochloride (DAB). The sections were photographed by Nikon microscopy and image analyses system. The expression levels of proteins were quantitated using Image Pro Plus 6.0 software (Media Cybernetics Inc., modern York, USA).

    Analysis of cell cycle

    After RL95-2 cells were incubated in serum-free medium overnight, cells were collected and rinsed with PBS twice. For cell cycle analysis, the cells were fixed in 70% pre-cold ethanol at 4 °C overnight and stained by PI. The DNA content was analyzed using a BD FACStar flux cytometer.

    Gas chromatography analysis of fatty acid compositions

    Lipids extraction from cells and tissues were performed according to a previous report41. The gas chromatography was done on an Agilent 7890A. Identification of components was done by comparison of retention times with those of PUFA analytical standard.

    Systematic metabolomic analysis of eicosanoids

    The RL95-2 cells were seeded in 15 cm dish 24 hours before adding various PUFA. After digesting with 0.25% trypsin, cells were washed twice by PBS and counted. 2 × 107 cells were needed for eicosanoid analysis in each sample. Eicosanoid extraction was performed according to a previous report54. Chromatographic separation involved an ACQUITY UPLC BEH C18 column consisting of ethylene-bridged hybrid particles. The metabolites were quantified by employ of a 5500 QTRAP hybrid triple quadrupole linear ion trap mass spectrometer equipped with a Turbo Ion Spray electrospray ionization (ESI) source.

    Statistical analysis

    Data are presented as mean ± SD of at least three independent experiments. Differences between groups were analyzed using Student’s t test or one-way ANOVA, and a plane of P < 0.05 was considered statistically significant.



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    Operations & Process Management: Principles & Practice for Strategic ImpactOperations & Process Management: Principles & Practice for Strategic Impact
    By Nigel Slack, Alistair Jones
    Publisher : Pearson (Feb 2018)
    ISBN10 : 129217613X
    ISBN13 : 9781292176130
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    Subject : Business & Economics
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    Computer Security: Principles and PracticeComputer Security: Principles and Practice
    By William Stallings, Lawrie Brown
    Publisher : Pearson (Aug 2017)
    ISBN10 : 0134794109
    ISBN13 : 9780134794105
    Our ISBN10 : 1292220619
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    Subject : Computer Science & Technology
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    Urban EconomicsUrban Economics
    By Arthur O’Sullivan
    Publisher : McGraw-Hill (Jan 2018)
    ISBN10 : 126046542X
    ISBN13 : 9781260465426
    Our ISBN10 : 1260084493
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    Subject : Business & Economics
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    Urban EconomicsUrban Economics
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    Publisher : McGraw-Hill (Jan 2018)
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    Our ISBN10 : 1260084493
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    Understanding BusinessUnderstanding Business
    By William G Nickels, James McHugh, Susan McHugh
    Publisher : McGraw-Hill (Feb 2018)
    ISBN10 : 126021110X
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    Our ISBN10 : 126009233X
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    Subject : Business & Economics
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    Understanding BusinessUnderstanding Business
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    Publisher : McGraw-Hill (May 2018)
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    Understanding BusinessUnderstanding Business
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    Publisher : McGraw-Hill (Jan 2018)
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    Our ISBN10 : 126009233X
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    Publisher : McGraw-Hill (Jan 2018)
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    Publisher : McGraw-Hill (Feb 2017)
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    Subject : Business & Economics, Communication & Media
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